Md Tawsif Ur Rashid

A highly motivated Master's graduate with research experience in Genetics, Plant Immunology, and Cell Biology. Passionate about Biotechnology, with a strong drive to contribute as a researcher in solving complex challenges in the field of Life Sciences. Committed to a disciplined and diligent approach, believing that, curiosity, hard work and perseverance form the foundation for meaningful scientific advancements.

1. Research Seed Grant Initiative Funding (RSGI) on 'Immunoassay based device for detection of UTI causing microorganisms from Urine samples in Bangladesh', 10/18/24, CURRENT  Co-Principal Investigator

• Rapid detection kit design to detect microorganisms from urine samples., Target organisms: E.coli & Klebsiella spp.,
• Principal Investigator: Dr. Munima Haque, Associate Professor, BRAC University
• Co-PI: Dr. Mahdi Moosa, Assistant Professor, BRAC University, Md Tawsif Ur Rashid, Lecturer, BRAC University, Subarna Hossain Turja, Lecturer, BRAC University

2. Characterization of Multivesicular Bodies (MVB) in yeast cells and analyzing exosome markers, 02/04/25- CURRENT

• Analyze the MVBs in yeast and their roles in possible protein secretions
• Techniques used: Culture of yeast (Media used: YPD, Malt & WL), Exosome Isolation protocol, Protein purification, SDS-PAGE and Western Blot
• Supervisor: Dr. Iftekhar Bin Naser

1. Master's Thesis: Investigating the role of PEN1-SYP122 syntaxins in mlo-based resistance and analyzing the mobility of membrane trafficking components and their involvement in pre-invasive immunity of Arabidopsis thaliana, 08/23/22- 06/22/23

• Identify the roles of PEN1/SYP122 syntaxins, Rab5 GTPases (ARA7-QL), and exosomes in pre-invasive immunity of Arabidopsis against powdery mildew
• Used confocal imaging techniques to check their localization, co-localization patterns, analyzed and quantified the fluorescence level of these proteins near the infection site/papilla using Las X Leica Microsystem, Gateway cloning method for cloning NahG into pK2GW7 destination vector, electroporation into E. coli, Agrobacterium-mediated transformation of the cloned plasmid into mlo6/12-pen1-syp122-fmo1 mutant line, colony PCR, gel electrophoresis, qPCR, restriction digestion
• Supervisor: Professor Hans Thordal-Christensen

2. Analyzing lung capacity for COVID-recovered patients and 3D bio-printing, 11/07/20-05/05/21

• Review paper, research on transplantation, transplantation efficiency, successful cases, 3D bioprinting, and its efficacy
• Analyzing if organs can be 3D printed successfully and finding cases., Analyzing if 3D bio-printed lungs can be transplanted., Graft vs Host reaction.
• Supervisor: Dr. Iftekhar Bin Naser

1. Professor Hans Thordal-Christensen,

Department of Plant and Environmental Sciences, University of Copenhagen, Denmark, Email: htc@plen.ku.dk

2. Dr. Munima Haque

Associate Professor, Department of Mathematics & Natural Sciences (MNS), Biotechnology Program, BRAC University

Email: munima.haque@bracu.ac.bd

3. Iftekhar Bin Naser, PhD

Associate Professor, Department of Mathematics and Natural Sciences (MNS), Biotechnology Program, BRAC University

Email: iftekhar.naser@bracu.ac.bd